Monday, 16 December 2013

                  Students Project

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Effect of different cooking methods on vegetable oxalate content.

Approximately 75% of all kidney stones are composed primarily of calcium oxalate, and hyperoxaluria is a primary risk factor for this disorder. Nine types of raw and cooked vegetables were analyzed for oxalate using an enzymatic method. There was a high proportion of water-soluble oxalate in most of the tested raw vegetables(spinach). Boiling markedly reduced soluble oxalate content by 30-87% and was more effective than steaming (5-53%) and baking (used only for potatoes, no oxalate loss). An assessment of the oxalate content of cooking water used for boiling and steaming revealed an approximately 100% recovery of oxalate losses. The losses of insoluble oxalate during cooking varied greatly, ranging from 0 to 74%. Because soluble sources of oxalate appear to be better absorbed than insoluble sources, employing cooking methods that significantly reduce soluble oxalate may be an effective strategy for decreasing oxaluria in individuals predisposed to the development of kidney stones.

The Dissolved oxalates estimated by KMno4 Titration method:
#take 20ml of solution and 20ml con.sulfuric acid and heat the content.
#the content titrated with 2M KMno4 solution
#Boiling method Solution contains more oxalates which is determined by calculation of how much KmnO4 is required?
#Hence the Boiling solution require 27ml KMnO4.
#The steamed Solution require 20ml KMnO4.
#So that In boiling method more oxalates dissolved in water which is right process now.
#ancient method from our bharat.(clay pot cooking).